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Identification of pathogen-inducible promoters largely lags behind cloning of the genes for disease resistance. Here, we cloned the soybean  GmaPPO12  gene and found that it was rapidly and strongly induced byPhytophthorasojaeinfection. Computational analysis revealed that its promoter contained many known  cis -elements, including several defense related transcriptional factor-binding boxes. We showed that the promoter could mediate induction of GUS expression upon infection in both transient expression assays inNicotianabenthamianaand stable transgenic soybean hairy roots. Importantly, we demonstrated that pathogen-induced expression of the  GmaPPO12  promoter was higher than that of the soybean  GmaPR1a  promoter. A progressive 5’ and 3’ deletion analysis revealed two fragments that were essential for promoter activity. Thus, the cloned promoter could be used in transgenic plants to enhance resistance to  phytophthora  pathogens, and the identified fragment could serve as a candidate to produce synthetic pathogen-induced promoters.

Identification and Functional Characterization of the Soybean GmaPPO12 Promoter Conferring Phytophthora sojae Induced Expression