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After recalling the basic relations relevant to both steady-state andtime-resolved fluorescence polarization, it is shown how the values ofsteady-state polarized intensities recorded experimentally usually need to becorrected for systematic effects and errors, caused by instrumentation andsample properties. A list of selected reference values of steady-statefluorescence anisotropy and polarization is given. Attention is also paid toanalysis of time-resolved fluorescence anisotropy data obtained by pulsefluorometry or phase and modulation fluorometry techniques. Recommendations forchecking the accuracy of measurements are provided together with a list ofselected time-resolved fluorescence anisotropy data as reported in theliterature.

Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)