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This study aimed to develop a simple method to make peripheral blood smear rich in mononuclear leucocytes with abundant cytoplasm. Such blood smear is essential for a simple and inexpensive micronucleus test. Blood samples were obtained from the Indonesian red cross, Central Jakarta. Various methods using combinations of hypotonic treatment andfrxationwere performed. Nine small scale methods 'rhowing good and consistent results were repeated on I0 indiviluals. Analysis on the ratio of intact cells, cell diameter andcytoplasmwidthwere done onmononuclear cells, Statistical analysis (baLancedAnova)were done using a Minitabprogram forWindows.Thebestmethod consistsofhypotonicteatmentusingplasm:aquabidest4:1,3:l,and2:l,smearingmethodresuyingin 2 slender smears, and slide ftxation using methanol: gLacial acetic acid 9: L

A simple method in preparing peripheral blood smear rich in mononuclear leucocytes with abundant cytoplasm for micronucleus test