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Hastuti D, Suranto, Setyono P. 2009. Variation of morphology, karyotype and protein band pattern of adenium (Adeniumobesum) varieties. Nusantara Bioscience 1: 78-83. The aim of this research to find out the Adenium obesum variation from six varieties,namely: obesum, cery, red lucas, red fanta , white bigben and harry potter based on morphology, karyotype, as well as protein bandingpattern. The chromosome preparation was made using semi-permanent squash method from the tip of root plant; while protein bandingpattern was made using SDS-PAGE method. Qualitative data included shape and color of the leave and flower described from eachvariety. Data were presented in morphometry and analyzed using ANOVA and then followed by DMRT with 5% of confidence levels,indicated significance difference. Protein banding pattern, the root, stem, leave and all organs were analyzed using Hierarchical ClusterAnalysis method with Average Linkage (between Groups) using SPSS 10.0. The result of research shows that the six A. obesumvarieties have morphological character with no variation of light green to dark green leave, not hairy, smooth leave bone, meanwhile forlight red to dark red flower crown color although some of them are white and the same funnel color, yellow. All varieties of A. obesumhave same number of chromosome, 2n = 22 and shows the difference ranging from 2.56 to 5.13 AŽÂ¼m. In the banding pattern formedqualitatively, there is variation among the six varieties.

Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties