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(Paralichthys olivaceus) elastase-like serine protease (PoElSp)  cDNA     , PoElSp  269   978 . PoElSp      RT-PCR  , ,       . lipopolysaccharide (LPS)    , 1 , 3 , PoElSp    . ,     LPS   1-24  . pro-mature PoElSp (proPoElSp)  cDNA pET32a     .   proPoElSp   gelatin zymography   Z-Phe-Arg-AMC   .      pH 7.5.  , PoElSp       .

susan haeyang gyoyuk yeon-gu/su'san haeyang gyoyug yeon'gu

Molecular Cloning, Expression Analysis and Enzymatic Characterization of Elastase-like Serine Protease from the Olive Flounder (Paralichthys olivaceus)