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1) Of 50 clinically normal calves 10 to 120days old, twenty were free from gross lesions in the lung. The remaining 30 claves had pulmonary lesions which were mild, moderate, and severe. 2) Haemophilus somnus was isolated from the lungs of 12 of 27 calves with moderate or severe gross lesions. Of the 27 calves, nine harbored Pasteurella multocida or other bacterial species, and twenty Ureaplasma spp. in the lung. No H. somnus or other bacteria were recovered from the lung of 23 calves with mild or no lesions. The younger the calves was obserred in the higher the isolation rate of H. somnus and Ureaplasma spp. 3) Of the 12 isolates, 7 were positive, 2 suspicious, and 3 negative for the slide agglutination test using antiserum prepared with the H. somnus reference strain. 4) The 30 calves, except one, with gross pulmonary lesions had antibody against H. somnus when examined by the tube agglutination test. The geometric mean was 52.0 in these 29. Of the 20 calves with no gross lesions, three were positive for antibody. The geometric mean was 25.0 in these 3.

Isolation of Haemophilus somnus and Detection of Antibody in Calves Raised in Tokachi, Hokkaido