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The objective of this study was to assess if larvae of Phylloicus spp. show any preference for leaves with or without microorganisms when building cases. Two experiments were realized at municipality of Presidente Figueiredo (Amazonas – Brazil), containing leaves treated with Hydrogen peroxide (H2O2), a compost that has an antimicrobial action, and another one with untreated leaves. In the first were observed the case-building by larvae of Phylloicus spp. And in the second was tested the preference by treated or untreated leaves. The larvae were monitored during 24 hours and to statistical analysis was used the t-test. Were not observed significant differences in the number of discs used by the larvae in the first experiment (p=0.24; t=-0.73) and no preference by type of leaf in the second experiment (p=0.41; t=0.23). Therefore, we do not recommend the use of hydrogen peroxide in studies that aim to eliminate microorganisms from decomposing leaves. Uso de Folhas Tratadas com Peroxido de Hidrogenio na Construcao de Abrigos por Phylloicus spp. (Trichoptera: Calamoceratidae) Resumo. O objetivo deste estudo foi avaliar se larvas de Phylloicus spp. possuem preferencia por folhas com ou sem micro-organismos para a construcao de abrigos. Dois experimentos foram realizados no municipio de Presidente Figueiredo (Amazonas – Brasil), contendo folhas tratadas com peroxido de hidrogenio (H2O2), composto que possui acao antimicrobiana, e folhas nao tratadas. No primeiro observaram-se a construcao de abrigos pelas larvas de Phylloicus spp. e no segundo testou-se a preferencia por folhas tratadas ou nao tratadas. As larvas foram monitoradas durante 24 horas e para a analise estatistica foi utilizado o teste-t. Nao foram observadas diferencas significativas no numero de discos usados pelas larvas no primeiro experimento (p=0,24; t=-0,73) e nem na preferencia pelo tipo de folha no segundo experimento (p=0,41; t=0,23). Portanto, nao recomendamos o uso do peroxido de hidrogenio em estudos realizados em laboratorio que visem a eliminacao de micro-organismos de folhas em decomposicao.

Use of Leaves Treated with Hydrogen Peroxide in Case-Building by Phylloicus spp. (Trichoptera: Calamoceratidae)