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pressor/cytotoxic T lymphocyte) and ƒ¿Leu3a (helper/inducer T lymphocyte) positive cells of peripheral blood E+cells in healthy adults were 80.0•}7.0%, 30.5•}7.5% and 53.0•}10.0%, respectively. The ratio of helper/suppressor T lymphocyte was 1.2•`2.2. These values were similar to the published values determined by immunofluorescence assay and complement-mediat ed cytotoxicity assay. In the malignant cell surface marker analysis, adult T-cell leukemia showed the phenotype of helper/inducer T lymphocytes (ƒ¿Leu4+, 2a-, 3a+); T-cell lymphoma, that of common thy mocyte (ƒ¿Leu 4+, 2a+, 3a+); acute lymphocytic leukemia, that of common ALL (ƒ¿cALL+, HLA DR+, ƒ¿Leu4-, 2a-, 3a-, S-Ig-). In the case of AMMoL, the percentage of MY 4 positive cells was found to be 56%, that of MY 7, 72% and that of MY 9, 77%. In addition, the simultaneous analysis of cytological features and monoclonal antibody-defined surface markers of rosette-form ing cells was achieved by preparing smears with cytochemical staining. This method is technically simple, commonly available and as sensitive as other techniques. Therefore, we consider it to be of value in routine practice and well-suited for use in the cha racterization of malignant cells.

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Study of monoclonal antibody rosetting technique for the enumeration of T lymphocyte subsets in peripheral blood and the characterization of leukemia and lymphoma cells