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We present a genome assembly from an individual female  Gymnoscelis rufifasciata  (the double-striped pug; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence is 352 megabases in span. The majority of the assembly (99.82%) is scaffolded into 32 chromosomal pseudomolecules, with the W and Z sex chromosomes assembled. The mitochondrial genome was also assembled, and is 15.4 kilobases in length.

The genome sequence of the double-striped pug, Gymnoscelis rufifasciata (Haworth, 1809)