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It is generally accepted that urinary hydroxyproline (H. P) is closely related to the turnover rate of collagen metabolism.1) This is why urinary H. P excretion can be used to estimate the growth potential of children.2),3) However there may be several other factors which also affect the amount of urinary H. P but are independent of growth potential. Investigations of these factors will be worthwhile in using urinary H. P excretion as a more reliable index of growth potential. Ziff and co-workers4) observed an increased excretion of H. P when it was added to the diet of human subjects in the form of gelatin. Their results suggest the possibility that a part of urinary H. P may originate from dietary proteins in the usual diet. On the other hand, procop et al.5) found that although ingestion of large amounts of gelatin increased urinary H. P, its urinary excretion did not decrease when H. P was eliminated from the normal diet, or even if subjects were placed on a protein-free diet. From these findings they reported that the daily excretion of H. P is practically independent of factors that influence the excretion of other amino acids. In this study, daily variation of urinary H. P excretion was investigated in five healthy men and women, putting emphasis on its relation to the daily protein intake. The daily variation of urinary creatinine, which is usually used as an index for the standardization of the concentration of various substances in a spot urine sample, was also investigated in the same five subjects.

The day to day variations of urinary hydroxyproline and creatinine excretions, and dietary protein intake.