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The NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During  Drosophila  midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that NTC-subunit Salsa, the  Drosophila  ortholog of human RNA helicase Aquarius, is rate-limiting for splicing of a subset of small first introns during oogenesis, including the first intron of  gurken . Germline depletion of Salsa and splice site mutations within  gurken  first intron impair both adult female fertility and oocyte dorsal–ventral patterning, due to an abnormal expression of Gurken. Supporting causality, the fertility and dorsal–ventral patterning defects observed after Salsa depletion could be suppressed by the expression of a  gurken  construct without its first intron. Altogether, our results suggest that one of the key rate-limiting functions of Salsa during oogenesis is to ensure the correct expression and efficient splicing of the first intron of  gurken  mRNA. Retention of  gurken  first intron compromises the function of this gene most likely because it undermines the correct structure and function of the transcript 5′UTR.

NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal–ventral patterning