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In  Staphylococcus aureus , a T-box riboswitch exists upstream of the  glyS  gene to regulate transcription of the sole glycyl-tRNA synthetase, which aminoacylates five tRNA Gly  isoacceptors bearing GCC or UCC anticodons. Subsequently, the glycylated tRNAs serve as substrates for decoding glycine codons during translation, and also as glycine donors for exoribosomal synthesis of pentaglycine peptides during cell wall formation. Probing of the predicted T-box structure revealed a long stem I, lacking features previously described for similar T-boxes. Moreover, the antiterminator stem includes a 42-nt long intervening sequence, which is  staphylococci -specific. Finally, the terminator conformation adopts a rigid two-stem structure, where the intervening sequence forms the first stem followed by the second stem, which includes the more conserved residues. Interestingly, all five tRNA Gly  isoacceptors interact with  S. aureus glyS  T-box with different binding affinities and they all induce transcription readthrough at different levels. The ability of both GCC and UCC anticodons to interact with the specifier loop indicates ambiguity during the specifier triplet reading, similar to the unconventional reading of glycine codons during protein synthesis. The  S. aureus glyS  T-box structure is consistent with the recent crystallographic and NMR studies, despite apparent differences, and highlights the phylogenetic variability of T-boxes when studied in a genome-dependent context. Our data suggest that the  S. aureus glyS  T-box exhibits differential tRNA selectivity, which possibly contributes toward the regulation and synchronization of ribosomal and exoribosomal peptide synthesis, two essential but metabolically unrelated pathways.

A <i>glyS</i> T-box riboswitch with species-specific structural features responding to both proteinogenic and nonproteinogenic tRNA<sup>Gly</sup> isoacceptors

A glyS T-box riboswitch with species-specific structural features responding to both proteinogenic and nonproteinogenic tRNAGly isoacceptors

A glyS T-box riboswitch with species-specific structural features responding to both proteinogenic and nonproteinogenic tRNA^Gly isoacceptors