Open AccessPeroxynitrite-Generating Species: Good Candidate Oxidants in Aqueous Extracts of Cigarette Smoke
Author(s) -
Yu Yamaguchi,
Satomi Kagota,
Jun Haginaka,
Masaru Kunitomo
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.82.78
Subject(s) - peroxynitrite , chemistry , nitrotyrosine , cigarette smoke , tyrosine , smoke , radical , pathogenesis , sidestream smoke , biochemistry , organic chemistry , immunology , medicine , toxicology , biology , superoxide , nitric oxide synthase , enzyme
Cigarette smoking is a well-known risk factor for atherosclerosis, but the mechanism of the adverse biological effect of smoking remains to be established. Cigarette smoke contains high concentrations of free radicals and oxidants. We show here that cigarette smoke extracts (CSE), prepared by bubbling the gas phase of smoke into phosphate-buffered saline, could convert tyrosine to 3-nitrotyrosine. The tyrosine nitration terminated 6 h after incubating tyrosine with CSE at 37 degrees C. These results indicate that the active oxidants in CSE are peroxynitrite-generating species like 3-morpholinosydnonimine (SIN-1), suggesting that they modify plasma lipoproteins and contribute to the pathogenesis of atherosclerosis.