Open AccessDrug Metabolism
Author(s) -
A.L. Allanson,
Anne Boyter,
J.N.A. Tettey,
Mark F. Cotton,
Jacqueline F. Sinclair,
Catherine Henderson,
Isabel Fernández de Castro Martín,
M.H. Grant
Publication year - 2007
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1211/002235707781850104
Subject(s) - drug metabolism , drug , pharmacology , medicine
Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes