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Background This study aimed to in vivo and in vitro evaluate the remineralization potential and shear bond strength (SBS) of experimentally prepared tri-calcium aluminate (C 3 A)-modified glass ionomer cements on sound and artificial caries-affected dentin (CAD). A pure tricalcium aluminate (C 3 A) phase prepared via solid state reaction at elevated temperature from chemically pure calcium carbonate and alumina, to formulate ceramic composite of composition: 75% C 3 A, 15% CaSO 4 ·2H 2 O and 10% Bi 2 O 3 . The influence of artificial saliva solution on the hydration characteristics and microstructure of the synthesized composite was investigated by X-ray diffraction (XRD) analysis, FTIR spectral analysis, pH determination and scanning electron microscope (SEM) in comparison with distilled water curing medium. Modified cements of C 3 A glass ionomers (C 3 A-CGIC) were prepared by addition of the experimentally prepared C 3 A to the powder component of the conventical glass ionomer cement (CGIC). Five and 10 wt% of C 3 A- CGICs powder were prepared and compared to CGIC. Cements were applied in prepared class V cavities in rabbits’ teeth either to sound or artificial CAD. All rabbits were killed after 15 days, and then, Ca and P wt% were evaluated at the cement–dentin interface using Energy-Dispersive X-ray Analysis. Specimens for SBS evaluation were prepared for the tested cements bonded either to sound or artificial CAD, then tested using universal testing machine. Results The XRD results indicate that there is an acceleration effect on the hydration reactions and decrease in the rate of conversion process of C 3 A phase composite due to the presence of free ions in saliva solution which was emphasized by the results of the IR spectral bands of the hydrated paste samples. The SEM micrographs showed a more-dense microstructure with large accumulations of aluminate hydrate crystals of samples cured under saliva solution. Results of the prepared C 3 A-CGICs showed that 10wt% C 3 A-GIC group had the highest statistically significant mean Ca, P wt% and SBS values on CAD compared to 5wt% C 3 A-GIC and CGIC. Conclusions 10 wt% C 3 A-GIC has a remineralizing effect on artificial CAD under in vivo conditions, plus its improved bonding to dentin compared to CGIC. Thus, it might be promising restorative/base with advanced remineralization potential and adequate bond strength to both sound dentin and CAD.

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Re-mineralization potential, shear bond strength and hydration characteristics of experimentally prepared tri-calcium aluminate phase-modified glass ionomer cement on sound and caries-affected dentin (An in vitro/in vivo study)