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For the first time, an aqueous extract of  Melilotus officinalis  was used to synthesize bimetallic silver selenide chalcogenide nanostructures (Ag 2 Se-NCs). The formation of NCs was confirmed and characterized by UV–visible and FTIR spectroscopy, SEM and TEM imaging, XRD and EDX crystallography, zeta potential (ZP) and size distribution (DLS). The bioactivities of biosynthesized Ag 2 Se-NCs, such as antibacterial, antibiofilm, antioxidant and cytotoxicity potentials, were then examined. Bio-based Ag 2 Se-NCs were successfully synthesized with mostly spherical shape in the size range of 20–40 nm. Additionally, the MIC and MBC values of Ag 2 Se-NCs against β-lactam-resistant  Pseudomonas aeruginosa  (ATCC 27853) were 3.12 and 50 µg/ml, respectively. The DPPH scavenging potential of Ag 2 Se-NCs in terms of IC 50  was estimated to be 58.52. Green-synthesized Ag 2 Se-NCs have been shown to have promising benefits and could be used for biomedical applications. Although the findings indicate promising bioactivity of Ag 2 Se-NCs synthesized by  M. officinalis  extract ( MO ), more studies are required to clarify the comprehensive mechanistic biological activities.

Bio-inspired silver selenide nano-chalcogens using aqueous extract of Melilotus officinalis with biological activities