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Flavone  C -arabinosides/xylosides are plant-originated glycoconjugates with various bioactivities. However, the potential utility of these molecules is hindered by their low abundance in nature. Engineering biosynthesis pathway in heterologous bacterial chassis provides a sustainable source of these  C -glycosides. We previously reported bifunctional  C -glucosyl/ C -arabinosyltransferases in  Oryza sativa  japonica and  O. sativa  indica, which influence the  C -glycoside spectrum in different rice varieties. In this study, we proved the  C -arabinosyl-transferring activity of rice  C -glycosyltransferases (CGTs) on the mono- C -glucoside substrate nothofagin, followed by taking advantage of specific CGTs and introducing heterologous UDP-pentose supply, to realize the production of eight different  C -arabinosides/xylosides in recombinant  E. coli . Fed-batch fermentation and precursor supplement maximized the titer of rice-originated  C -arabinosides to 20–110 mg/L in an  E. coli  chassis. The optimized final titer of schaftoside and apigenin di- C -arabinoside reached 19.87 and 113.16 mg/L, respectively. We demonstrate here the success of de novo bio-production of  C -arabinosylated and  C -xylosylated flavones by heterologous pathway reconstitution. These results lay a foundation for further optimal manufacture of complex flavonoid compounds in microbial cell factories.Supplementary Information  The online version contains supplementary material available at 10.1186/s40643-021-00404-3.

De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases