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Enhancing cellulase production in  Trichoderma reesei  is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in  T. reesei . In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16.  T. reesei  transformants T XYR1VP , T ACE2VP , and T ACE1VP  showed improved cellulase and/or xylanase production. T XYR1VP  has a cellulase-free phenotype but with significantly elevated xylanase production. Xylanase I and xylanase II activities [U/(mg biomass)] increased by 51% and 80%, respectively, in T XYR1VP  in comparison with parental strain RUT C30. The filter paper activity of T ACE2VP  in the Avicel-based medium increased by 52% compared to that of RUT C30. In the Avicel-based medium, T ACE1VP  manifested an 80% increase in FPase activity and a 50% increase in xylanase activity as compared to those of RUT C30. Additionally, when pretreated corn stover was hydrolyzed, crude enzymes produced from T ACE1VP  yielded a greater glucose release than did the enzymes produced by parental strain RUT C30.Electronic supplementary material  The online version of this article (10.1186/s40643-018-0226-4) contains supplementary material, which is available to authorized users.

Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30