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Botryococcus braunii is difficult to cultivate and has a limited amount of substantive scale-up and productivity assessments with conventionally suspended cultivation systems, such as open pond or closed photobioreactors. The biomass concentrations of cultivated microalgal biofilms are much higher than those of suspension cultures, and the attached microalgal cells are easily separated from cultivation media. However, studies on the attached cultivation conditions for B. braunii have been rarely performed.

bioresources and bioprocessing

Effects of seed age, inoculum density, and culture conditions on growth and hydrocarbon accumulation of Botryococcus braunii SAG807-1 with attached culture