English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This study aimed to compare the functional capacity and gene expression profile of monocyte-derived dendritic cells (MD-DCs) in HLA-B27 +  axial spondyloarthritis (SpA) patients and healthy controls.    Methods  MD-DCs were differentiated with interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) for seven days, starting from purified CD14 +  monocytes and stimulated with lipopolysaccharide (LPS) for six and twenty four hours. Their capacity to stimulate allogeneic CD4 +  T cells from unrelated healthy donor was tested. Transcriptomic study was performed with Affymetrix HuGene 1.0 ST microarrays. Gene expression levels were compared between patients and controls using a multivariate design under a linear model (LIMMA). Real-time quantitative PCR (qRT-PCR) was performed for validation of the most striking gene expression differences.    Results  The stimulatory capacity of allogeneic CD4 +  T cells by MD-DCs from SpA patients was decreased. Transcriptomic analysis revealed 81 genes differentially expressed in MD-DCs between SpA patients and controls ( P  <0.01 and fold-change <0.66 or >1.5). Four selected genes were validated by qRT-PCR:  ADAMTS15, CITED2, F13A1  and  SELL . Expression levels of  ADAMTS15  and  CITED2,  encoding a metallopeptidase and a transcription factor, respectively, were inversely correlated with each other (R = 0.75,  P  = 0.0003). Furthermore,  in silico  analysis identified several genes of the Wnt signaling pathway having expression co-regulated with  CITED2 .    Conclusion  This study revealed altered function and gene expression pattern in MD-DCs from HLA-B27 +  axial SpA. Co-expression study showed an inverse correlation between  ADAMTS15  and  CITED2 . Moreover, the Wnt signaling pathway appeared as deregulated in SpA MD-DCs, a finding which may be connected to Th17-driven inflammatory responses.    Electronic supplementary material  The online version of this article (doi:10.1186/s13075-014-0417-0) contains supplementary material, which is available to authorized users.

arthritis research and therapy

Monocyte-derived dendritic cells from HLA-B27+ axial spondyloarthritis (SpA) patients display altered functional capacity and deregulated gene expression