English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Loop-mediated isothermal amplification (LAMP) has been widely used to diagnose various infectious diseases. Malaria is a globally distributed infectious disease attributed to parasites in the genus  Plasmodium . It is known that persons infected with  Plasmodium vivax  and  P. ovale  are prone to clinical relapse of symptomatic blood-stage infections. LAMP has not previously been specifically evaluated for its diagnostic performance in detecting  P. ovale  in an epidemiological study, and no commercial LAMP or rapid diagnostic test (RDT) kits are available for specifically diagnosing infections with  P. ovale .    Methods  An assay was designed to target a portion of mitochondrial DNA ( mtDNA ) among  Plasmodium  spp., the five human  Plasmodium  species and two other assays were designed to target the nuclear 18S ribosomal DNA gene ( 18S rDNA ) of either  P. vivax  or  P. ovale  for differentiating the two species. The sensitivity of the assays was compared to that of nested PCR using defined concentrations of plasmids containing the target sequences and using limiting dilutions prepared from clinical isolates derived from Chinese workers who had become infected in Africa or near the Chinese border with Myanmar.    Results  The results showed that 10 2  copies of the mitochondrial target or 10 2  and 10 3  copies of  18S rDNA  could be detected from  Plasmodium  spp.,  P. vivax  and  P. ovale , respectively. In 279 clinical samples, the malaria Pan  mtDNA  LAMP test performed well when compared with a nested PCR assay (95% confidence interval [CI] sensitivity 98.48–100%; specificity 90.75–100%). When diagnosing clinical cases of infection with  P. vivax , the  18S rDNA  assay demonstrated an even great sensitivity (95.85–100%) and specificity (98.1–100%). The same was true for clinical infections with  P. ovale  (sensitivity 90.76–99.96%; specificity 98.34–100%). Using plasmid-positive controls, the limits of detection of Malaria Pan,  18S rDNA P. vivax  and  18S rDNA P. ovale  LAMP were 100-, 100- and tenfold lower than those of PCR, respectively.    Conclusion  The novel LAMP assays can greatly aid the rapid, reliable and highly sensitive diagnosis of infections of  Plasmodium  spp. transmitted among people ,  including  P. vivax  and  P. ovale , cases of which are most prone to clinical relapse.    Graphic abstractSupplementary Information  The online version contains supplementary material available at 10.1186/s13071-021-04764-9.

parasites and vectors

Loop-mediated isothermal amplification (LAMP) assays targeting 18S ribosomal RNA genes for identifying P. vivax and P. ovale species and mitochondrial DNA for detecting the genus Plasmodium