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Background  The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria.    Methods    Staphylococcus  phage JD007 was characterized and its complete genome sequence analysed.    Results    Staphylococcus  phage JD007 was classified as belonging to the  Myoviridae  family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5–11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest  Staphylococcus  phages of  Myoviridae . No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of  S. aureus  isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit  S. aureus  growth at a multiplicity of infection of 0.1.    Conclusions  The results suggested that  Staphylococcus  phage JD007 can potentially be used in phage therapy or for the detection of  S. aureus .    Electronic supplementary material  The online version of this article (doi:10.1186/s12985-017-0701-0) contains supplementary material, which is available to authorized users.

virology journal

Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China