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Background  Glycosylation of flavonoids is a promising approach to improve the pharmacokinetic properties and biological activities of flavonoids. Recently, many efforts such as enzymatic biocatalysis and the engineered  Escherichia coli  biotransformation have increased the production of flavonoid glucosides. However, the low yield of flavonoid glucosides can not meet the increasing demand for human medical and dietary needs.  Saccharomyces cerevisiae  is a generally regarded as safe (GRAS) organism that has several attractive characteristics as a metabolic engineering platform for the production of flavonoid glucosides. However, endogenous glucosidases of  S.  cerevisiae  as a whole-cell biocatalyst reversibly hydrolyse the glucosidic bond and hinder the biosynthesis of the desired products. In this study, a model flavonoid, scutellarein, was used to exploit how to enhance the production of flavonoid glucosides in the engineered  S.  cerevisiae .    Results  To produce flavonoid glucosides, three flavonoid glucosyltransferases (SbGTs) from  Scutellaria baicalensis  Georgi were successfully expressed in  E. coli,  and their biochemical characterizations were identified. In addition, to synthesize the flavonoid glucosides in whole-cell  S. cerevisiae , SbGT34 was selected for constructing the engineering yeast. Three glucosidase genes ( EXG1 ,  SPR1 ,  YIR007W ) were knocked out using homologous integration, and the  EXG1  gene was determined to be the decisive gene of  S. cerevisiae  in the process of hydrolysing flavonoid glucosides. To further enhance the potential glycosylation activity of  S. cerevisiae , two genes encoding phosphoglucomutase and UTP-glucose-1-phosphate uridylyltransferase involved in the synthetic system of uridine diphosphate glucose were over-expressed in  S. cerevisiae . Consequently, approximately 4.8 g (1.2 g/L) of scutellarein 7- O -glucoside (S7G) was produced in 4 L of medium after 54 h of incubation in a 10-L fermenter while being supplied with ~3.5 g of scutellarein.    Conclusions  The engineered yeast harbouring SbGT with a deletion of glucosidases produced more flavonoid glucosides than strains without a deletion of glucosidases. This platform without glucosidase activity could be used to modify a wide range of valued plant secondary metabolites and to explore of their biological functions using whole-cell  S. cerevisiae  as a biocatalyst.    Electronic supplementary material  The online version of this article (doi:10.1186/s12934-016-0535-2) contains supplementary material, which is available to authorized users.

Engineering Saccharomyces cerevisiae with the deletion of endogenous glucosidases for the production of flavonoid glucosides