English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Leaf senescence is a genetically controlled degenerative process intimately linked to phosphate homeostasis during plant development and responses to environmental conditions. Senescence is accelerated by phosphate deficiency, with recycling and mobilization of phosphate from senescing leaves serving as a major phosphate source for sink tissues. Previously,  miR827  was shown to play a significant role in regulating phosphate homeostasis, and induction of its expression was also observed during Arabidopsis leaf senescence. However, whether shared mechanisms underlie potentially common regulatory roles of  miR827  in both processes is not understood. Here, we dissect the regulatory machinery downstream of  miR827 .    Results  Overexpression or inhibited expression of  miR827  led to an acceleration or delay in the progress of senescence, respectively. The transcriptional regulator GLABRA1 enhancer-binding protein (GeBP)-like ( GPLα ) gene was identified as a possible target of  miR827 .  GPLα  expression was elevated in  miR827 -suppressed lines and reduced in  miR827 -overexpressing lines. Furthermore, heterologous co-expression of pre- miR827  in tobacco leaves reduced  GPLα  transcript levels, but this effect was eliminated when pre-miR827 recognition sites in  GPLα  were mutated.  GPLα  expression is induced during senescence and its inhibition or overexpression resulted in senescence acceleration and inhibition, accordingly. Furthermore,  GPLα  expression was induced by phosphate deficiency, and overexpression of  GPLα  led to reduced expression of  phosphate transporter 1  genes, lower leaf phosphate content, and related root morphology. The encoded GPLα protein was localized to the nucleus.    Conclusions  We suggest that  MiR827  and the transcription factor GPLα may be functionally involved in senescence and phosphate homeostasis, revealing a potential new role for  miR827  and the function of the previously unstudied GPLα. The close interactions between senescence and phosphate homeostasis are further emphasized by the functional involvement of the two regulatory components,  miR827  and GPLα, in both processes and the interactions between them.

Downregulation of GeBP-like α factor by MiR827 suggests their involvement in senescence and phosphate homeostasis