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Background  Productions of metallo-β-lactamases enzymes are the most common mechanism of antibiotic resistance to all beta-lactam classes (except monobactams) in  Acinetobacter baumannii . MBLs are usually associated with gene cassettes of integrons and spread easily among bacteria. The current study was performed to detect the genes encoding MBLs and integron structures in  A. baumannii  isolates from burn patients.    Methods  This study was performed on 106 non-duplicate  A. baumannii  isolates from burn patients referred to Shahid Motahari Hospital in Tehran. Antibiotic susceptibility of  A. baumannii  isolates was performed using disk diffusion and broth microdilution method in accordance with the CLSI guidelines. The presence of class 1 integron and associated gene cassettes as well as MBLs-encoding genes including  bla  VIM , and  bla  IMP  were investigated using PCR and sequencing techniques.    Results  In this cross-sectional study all (100%) of the  A. baumannii  isolates examined were multidrug resistant. All isolates were sensitive to colistin and simultaneously all were resistant to imipenem. PCR assays showed the presence of  bla  VIM  and  bla  IMP  genes in 102 (96.2%) and 62 (58.5%) isolates of  A. baumannii  respectively. In addition, 62 (58.5%) of the  A. baumannii  isolates carried integron class 1, of which 49 (79.0%) were identified with at least one gene cassette. Three types of integron class 1 gene cassettes were identified including:  arr2 ,  cmlA5 ,  qacE1  (2300 bp);  arr-2 ,  ereC ,  aadA1 ,  cmlA7 ,  qacE1  (4800 bp); and  aac(3)-Ic ,  cmlA5  (2250 bp).    Conclusion  A high prevalence of MBLs genes, especially  bla  VIM , was identified in the studied MDR  A. baumannii  isolates. In addition, most of the strains carried class 1 integrons. Furthermore, the gene cassettes arrays of integrons including  cmlA5  and  cmlA7  were detected, for the first time, in  A. baumannii  strains in Iran.

Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients