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Background   Trueperella pyogenes  is one of the most clinically imperative bacteria responsible for severe cases of mastitis and metritis, particularly in postpartum dairy cows. The bacterium has emergence of antibiotic resistance and virulence characters. The existing research was done to apprise the phenotypic and genotypic evaluation of antibiotic resistance and characterization of virulence factors in the  T. pyogenes  bacteria of bovine mastitis and metritis in postpartum cows.    Methods  Two-hundred and twenty-six bovine mastitic milk and 172 uterine swabs were collected and transferred to laboratory. Samples were cultured and  T. pyogenes  isolates were subjected to disk diffusion and DNA extraction. Distribution of virulence and antibiotic resistance genes was studied by PCR.    Results  Thirty-two out of 226 (14.15%) mastitic milk and forty-one out of 172 (23.83%) uterine swab samples were positive for  T. pyogenes . Isolates of mastitic milk harbored the highest prevalence of resistance toward gentamicin (100%), penicillin (100%), ampicillin (90.62%), amoxicillin (87.50%) and trimethoprim-sulfamethoxazole (87.50%), while those of metritis harbored the highest prevalence of resistance toward ampicillin (100%), amoxicillin (100%), gentamicin (97.56%), penicillin (97.56%) and cefalexin (97.56%).  AacC ,  aadA1 ,  aadA2  and  tetW  were the most generally perceived antibiotic resistance genes. All bacteria harbored  plo  (100%) and  fimA  (100%) virulence factors.  NanP ,  nanH ,  fimC  and  fimE  were also the most generally perceived virulence factors.    Conclusions  All bacteria harbored  plo  and  fimA  virulence factors which showed that they can use as specific genetic markers with their important roles in pathogenicity of  T. pyogenes  bacteria. Phenotypic pattern of antibiotic resistance was confirmed by genotypic characterization of antibiotic resistance genes.

Phenotypic and molecular characterization of antimicrobial resistance in Trueperella pyogenes strains isolated from bovine mastitis and metritis