English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Pythium insidiosum  is a member of the oomycetes class of aquatic fungus-like microorganisms. It can infect humans and animals through skin wounds and the eyes, causing pythiosis, an infectious disease with high morbidity and mortality rates. Antifungal agents are ineffective as pythiosis treatments because ergosterol, the target site of most antifungal agents, is not found in the  P. insidiosum  cytoplasmic membrane. The best choice for treatment is surgical removal of the infected organ. While natural plant products or secretory substances from bacterial flora have exhibited in vitro anti- P. insidiosum  activity, their mechanism of action remains unknown. Therefore, this study hypothesized that the mechanism of action could be related to changes in  P. insidiosum  biochemical composition (such as lipid, carbohydrate, protein or nucleic acid) following exposure to the inhibitory substances. The biochemical composition of  P. insidiosum  was investigated by Synchrotron radiation-based Fourier-transform infrared (FTIR) microspectroscopy.    Results  Fraction No.6 from the crude extract of  P. stutzeri  ST1302, fraction No.1 from the crude extract of  K. pneumoniae  ST2501 and xanthyletin were used as anti- P. insidiosum  substances, with MFCs at 3.125, 1.57–1.91, 0.003 mg/ml, respectively. The synchrotron FTIR results show that the deconvoluted peak distributions in the amide I, amide II, and mixed regions were significantly different between the treatment and control groups.    Conclusions  Xanthyletin and the secondary metabolites from  P. stutzeri  ST1302 and  K. pneumoniae  ST2501 exerted anti- P. insidiosum  activity that clearly changed the proteins in  P. insidiosum . Further study, including proteomics analysis and in vivo susceptibility testing, should be undertaken to develop a better understanding of the mechanism of anti- P. insidiosum  activity.

Analysis of xanthyletin and secondary metabolites from Pseudomonas stutzeri ST1302 and Klebsiella pneumoniae ST2501 against Pythium insidiosum