English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  To facilitate infection,  Xanthomonas oryzae  pv.  oryzae  ( Xoo ), the bacterial blight pathogen of rice, needs to degrade hydrogen peroxide (H 2 O 2 ) generated by the host defense response via a mechanism that is mediated by the transcriptional regulator OxyR. The catalase (CAT) gene  catB  has previously been shown to belong to the OxyR regulon in  Xoo . However, its expression patterns and function in H 2 O 2  detoxification and bacterial pathogenicity on rice remain to be elucidated.    Results  The  catB  gene encodes a putative catalase and is highly conserved in the sequenced strains of  Xanthomonas  spp. β-galactosidase analysis and electrophoretic mobility shift assays (EMSA) showed that OxyR positively regulated the transcription of  catB  by directly binding to its promoter region. The quantitative real-time PCR (qRT-PCR) assays revealed that the expression levels of  catB  and  oxyR  were significantly induced by H 2 O 2 . Deletion of  catB  or  oxyR  drastically impaired bacterial viability in the presence of extracellular H 2 O 2  and reduced CAT activity, demonstrating that CatB and OxyR contribute to H 2 O 2  detoxification in  Xoo . In addition, Δ catB  and Δ oxyR  displayed shorter bacterial blight lesions and reduced bacterial growth in rice compared to the wild-type stain, indicating that CatB and OxyR play essential roles in the virulence of  Xoo .    Conclusions  Transcription of  catB  is enhanced by OxyR in response to exogenous H 2 O 2 . CatB functions as an active catalase that is required for the full virulence of  Xoo  in rice.    Electronic supplementary material  The online version of this article (doi:10.1186/s12866-016-0887-0) contains supplementary material, which is available to authorized users.

OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae