English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Regardless of sanitation practices implemented to reduce  Salmonella  prevalence in poultry processing plants, the problem continues to be an issue. To gain an understanding of the attachment mechanism of  Salmonella  to broiler skin, a bioluminescent-based mutant screening assay was used. A random mutant library of a field-isolated bioluminescent strain of  Salmonella enterica  serovar Kentucky was constructed. Mutants’ attachment to chicken skin was assessed in 96-well plates containing uniform 6 mm diameter pieces of circular chicken skin. After washing steps, mutants with reduced attachment were selected based on reduced bioluminescence, and transposon insertion sites were identified.    Results  Attachment attenuation was detected in transposon mutants with insertion in genes encoding flagella biosynthesis, lipopolysaccharide core biosynthesis protein, tryptophan biosynthesis, amino acid catabolism pathway, shikimate pathway, tricarboxylic acid (TCA) cycle, conjugative transfer system, multidrug resistant protein, and ATP-binding cassette (ABC) transporter system. In particular, mutations in  S . Kentucky flagellar biosynthesis genes ( flgA ,  flgC ,  flgK ,  flhB,  and  flgJ ) led to the poorest attachment of the bacterium to skin.    Conclusions  The current study indicates that attachment of  Salmonella  to broiler skin is a multifactorial process, in which flagella play an important role.

Identification of Salmonella enterica serovar Kentucky genes involved in attachment to chicken skin