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Background  A TaqMan real-time PCR assay targeting the  Anaplasma  citrate synthase gene,  gltA , was developed and used for detection of  Anaplasma phagocytophilum  in 765  Ixodes ricinus  ticks collected from dogs and cats in northern Norway ( n  = 669) and Telemark county in southern Norway ( n  = 96).    Results  Among the ticks from northern Norway the prevalence of  A. phagocytophilum  was 3.0 %, while the prevalence in southern Norway was 2.1 % ( p  = 0.63). The  gltA  PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies.    Conclusion  This is the first report of  A. phagocytophilum  occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.

bmc microbiology

Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA