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Background  Sheath blight disease (ShB) is a destructive disease affecting rice production. Previously, we have reported that Loose Plant Architecture 1 (LPA1) promotes resistance to ShB. However, the mechanisms by which LPA1 confers resistance against this disease have not been extensively investigated. Notably, interactors that regulate LPA-1 activity remain elusive.    Findings  In this study, we identified the interaction of kinesin-like protein (KLP) with LPA1 in the nucleus of rice cells by yeast two-hybrid, bimolecular fluorescent complimentary (BiFC), and co-immunoprecipitation (co-IP) assays. To investigate the role of  KLP  in promoting resistance to ShB, wild-type,  klp  mutant, and  KLP  overexpressor ( KLP OX ) rice plants were inoculated with  Rhizoctonia solani  AG1-IA. The results indicated that, compared with the wild-type control,  klp  mutants were more susceptible while  KLP OX  plants were less susceptible to ShB. Since LPA1 transcriptionally activates  PIN-FORMED 1a (PIN1a) , we examined the expression of 8 related  PIN  genes. The results showed that only the expression of  PIN1a  and  PIN3b  coincided with  KLP  expression levels. In addition, a chromatin immunoprecipitation (ChIP) assay showed that KLP bound directly to the promoter region of  PIN1a  but not of  PIN3b . Transient expression assays confirmed that LPA1 and KLP transcriptionally activate  PIN1a , and that coexpression of KLP and LPA1 had an additive effect on the activation of  PIN1a , suggesting that KLP enhances LPA1 transcriptional activation activity.    Conclusions  Taken together, our results show that KLP is a novel LPA1 interactor that promotes resistance of rice to ShB.

Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease