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Efficient bio-ethanol production from napiegrass ( Pennisetum purpureum  Schumach) was investigated. A low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass was subjected to simultaneous saccharification and co-fermentation (SSCF), which was performed at 36°C using  Escherichia coli  KO11,  Saccharomyces cerevisiae , cellulase, and xylanase. It was found that use of xylanase as well as the LMAA-pretreatment was effective for the SSCF. After the SSCF for 96 h, the ethanol yield reached 74% of the theoretical yield based on the glucan (397 mg g -1 ) and xylan (214 mg g -1 ) occurring in dry powdered LMAA-pretreated napiergrass.

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Bio-ethanol production through simultaneous saccharification and co-fermentation (SSCF) of a low-moisture anhydrous ammonia (LMAA)-pretreated napiegrass (Pennisetum purpureum Schumach)