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Conjugated linoleic acid l-menthyl ester was hydrolyzed in water by the lipase from  Candida rugosa  with the addition of an organic solvent. The degree of hydrolysis (yield) greatly improved when a tertiary alcohol, such as  t -butyl alcohol, was added. However, the addition of a less polar solvent, such as hexane, decreased the degree of hydrolysis, and some water-miscible solvents, such as acetone, caused inactivation of the lipase. With the addition of  t -butyl alcohol, the reaction mixture formed a one- or two-phase system, and the mixing ratio of substrates and  t -butyl alcohol determined the number of phases. Although the degree of hydrolysis at 10 d was higher in the one-phase system, the initial reaction rate was generally lower. Meanwhile, the reaction was much faster in the two-phase system while maintaining a moderate degree of hydrolysis.

Promotion of the lipase-catalyzed hydrolysis of conjugated linoleic acid l-menthyl ester by addition of an organic solvent