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Background   Vibrio cholerae  is the causative organism of waterborne disease, cholera.  V. cholerae  has caused many epidemics and pandemics of cholera for many years. In this study,  V. cholerae  recovered from edible ice were investigated for their genetic diversity using Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and Repetitive Extragenic Palindromic (REP) PCR. Isolation was done using selective medium and the presumptive isolates were confirmed through biochemical and serological assays.    Results  Seventy-five isolates of  V. cholerae  were recovered from ice samples collected from different locations of Jakarta. Specifically, 19 of them were identified as O1 serotype, 16 were Ogawa, 3 isolates were Inaba and the remaining isolates were non-O1. The fingerprinting profiles of  V.cholerae  isolated from ice samples were very diverse.    Conclusion  This result showed that the ERIC sequence is more informative and discriminative than REP sequence for analysis of  V. cholerae  diversity.

Use of REP- and ERIC-PCR to reveal genetic heterogeneity of Vibrio cholerae from edible ice in Jakarta, Indonesia