English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Expression of  Xist , the master regulator of X chromosome inactivation, is extinguished in pluripotent cells, a process that has been linked to programmed X chromosome reactivation. The key pluripotency transcription factors Nanog, Oct4 and Sox2 are implicated in  Xist  gene extinction, at least in part through binding to an element located in  Xist  intron 1. Other pathways, notably repression by the antisense RNA  Tsix , may also be involved.    Results  Here we employ a transgene strategy to test the role of the intron 1 element and  Tsix  in repressing  Xist  in ES cells. We find that deletion of the intron 1 element causes a small increase in  Xist  expression and that simultaneous deletion of the antisense regulator  Tsix  enhances this effect.    Conclusion  We conclude that  Tsix  and pluripotency factors act synergistically to repress  Xist  in undifferentiated embryonic stem cells. Double mutants do not exhibit maximal levels of  Xist  expression, indicating that other pathways also play a role.

Pluripotency factor binding and Tsix expression act synergistically to repress Xist in undifferentiated embryonic stem cells