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Background  Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.    Findings  We describe here protocols which adapt baculovirus generation into 96-well format.    Conclusion  The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells  in vitro  and  in vivo .

A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells