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Background  We report the expression pattern of  5S rDNA  in the eggs of water frogs  Rana lessonae ,  Rana ridibunda  and  Rana esculenta  using the quantitative real-time PCR. This kind of research had never been performed before.    Results   5S rDNA  relative expression of the  Rana ridibunda  oocytes is approximately six times higher in comparison to the  Rana lessonae  oocytes. The oocytes of the investigated  Rana esculenta  frogs, in respect of  5S rDNA  relative expression ratio, were very similar to the  Rana ridibunda  oocytes.    Conclusion  We suggest the possibility of using  5S rDNA  as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the  Rana lessonae ,  Rana ridibunda  and  Rana esculenta  oocytes.

Expression of 5S rDNA in the oocytes of water frogs