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Background  The cytosol of amoeba cells controls the membrane deformation during their motion  in vivo . To investigate such ability of the cytosol of amoeba cell,  Dictyostelium discoideum  ( Dictyostelium ),  in vitro , we used lipids extracted from  Dictyostelium  and commercially available phospholipids, and prepared substrate-supported lipid membrane patches on the micrometer scale by spin coating.    Results  We found that the spin coater holder, which has pores (pore size = 3.1 mm) of negative pressure to hold the cover glass induced the concave surface of the cover glass. The membrane lipid patches were formed at each position in the vicinity of the holder pores and their sizes were in the range of 2.7 to 3.2 × 10 4  μm 2 . After addition of the cytosol extracted from  Dictyostelium  to the lipid membrane patches, through time-lapse observation with a confocal laser scanning fluorescence microscope, we observed an autonomous buckling of the  Dictyostelium  lipid patches and localized behaviours of proteins found within.    Conclusion  The current method serves as the novel technique for the preparation of film patches in which the positions of patches are controlled by the holder pores without fabricating, modifying, and arranging the chemical properties of the solution components of lipids. The findings imply that lipid-binding proteins in the cytosol were adsorbed and accumulated within the  Dictyostelium  lipid patches, inducing the transformation of the cell-sized patch.    Electronic supplementary material  The online version of this article (doi:10.1186/1754-1611-9-3) contains supplementary material, which is available to authorized users.

Autonomous buckling of micrometer-sized lipid-protein membrane patches constructed by Dictyostelium discoideum