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Many of the gene regulatory networks used within the field of synthetic biology have extensively employed the AraC and LacI inducible transcription factors. However, there is no  Escherichia coli  strain that provides a proper background to use both transcription factors simultaneously. We have engineered an improved  E. coli  strain by knocking out the endogenous  lacI  from a strain optimal for AraC containing networks, and thoroughly characterized the strain both at molecular and functional levels. We further show that it enables the gradual and independent induction of both AraC and LacI in a simultaneous manner. This construct will be of direct use for various synthetic biology applications.

An improved Escherichia coli strain to host gene regulatory networks involving both the AraC and LacI inducible transcription factors