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Background  Polygoni Multiflori Radix,  He-Shou-Wu  in Chinese, is a widely used traditional Chinese medicine. Clinically, water decoction is the major application form of  He-Shou-Wu . Therefore, simultaneous determination of bioactive compounds in water extract is very important for its quality control.    Results  A pressurized liquid extraction and short-end injection micellar electrokinetic chromatography (MEKC) were first developed for simultaneous determination of seven hydrophilic bioactive compounds in water extract of  He-Shou-Wu . The influence of parameters, such as pH, concentration of phosphate, SDS and HP-β-CD, capillary temperature and applied voltage, on the analysis were carefully investigated. Optimum separation was obtained within 14 min by using 50 mM phosphate buffer containing 90 mM SDS and 2% (m/v) HP-β-CD (pH 2.5) at 15 kV and 20°C. All calibration curves showed good linearity ( R   2  >0.9978) within test ranges. The overall LOD and LOQ were lower than 2.0 μg/mL and 5.5 μg/mL, respectively. The RSDs for intra- and inter-day of seven analytes were less than 3.2% and 4.6%, and the recoveries were 97.0%-104.2%.    Conclusion  The validated method was successfully applied to the analysis of  He-Shou-Wu  samples, which is helpful for its quality control.

chemistry central journal

Simultaneous determination of seven hydrophilic bioactive compounds in water extract of Polygonum multiflorumusing pressurized liquid extraction and short-end injection micellar electrokinetic chromatography