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Background   Dichelobacter nodosus  is the causative agent of footrot in sheep. The survival of the bacterium in soil is of importance for the epidemiology of the disease. The investigation evaluates the survival of  D. nodosus  in soil with and without added hoof powder stored under different temperatures.    Results  An experimental setup was used with bacteriological culture and real-time polymerase chain reaction (PCR), and the results indicate that the bacteria can survive in soil for longer time than previously expected. The survival time was found to be dependent on temperature and the addition of hoof powder to the soil, with the longest survival time estimated to be 24 days in soil samples with hoof powder stored at 5°C.    Conclusion  Our findings indicate that the survival time of  D. nodosus  and its ability to infect susceptible sheep on pasture under different climatic conditions should be studied further.

An evaluation of the ability of Dichelobacter nodosus to survive in soil