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<p>Abstract</p> <p>To understand the hormonal coordination of the antagonism between molting and reproduction in crustaceans, the terminally anecdysial mature female <it>Callinectes sapidus </it>was used as a model. The regulatory roles of crustacean hyperglycemic hormone (CHH) and molt-inhibiting hormone (MIH) in vitellogenesis were examined. A competitive specific RIA was used to measure the levels of MIH and CHH in the hemolymphs of mature females at pre- and mid- vitellogenic stages, and their effects on vitellogenesis at early (early 2, E2) and mid vitellogenesis (3) stages were determined <it>in vitro</it>. A hepatopancreas fragments incubation system was developed and the levels of vitellogenin (VtG), as well as <it>VtG </it>mRNA and <it>heterogeneous nuclear (hn)VtG </it>RNA were determined using RIA or QPCR, respectively. MIH titers were four times higher at mid-vitellogenesis than at pre-vitellogenesis, while CHH levels in the hemolymph were constant. In the <it>in vitro </it>incubation experiments, MIH increased both <it>VtG </it>mRNA levels and secretion at ovarian stage 3. At stage E2, however, MIH resulted in a mixed response: downregulation of <it>VtG </it>mRNA and upregulation of <it>hnVtG </it>RNA. CHH had no effect on any of the parameters. Actinomycin D blocked the stimulatory effects of MIH in stage 3 animals on <it>VtG </it>mRNA and VtG, while cycloheximide attenuated only VtG levels, confirming the MIH stimulatory effect at this stage. MIH is a key endocrine regulator in the coordination of molting and reproduction in the mature female <it>C. sapidus</it>, which simultaneously inhibits molt and stimulates vitellogenesis.</p

saline systems

Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 1: an ovarian stage dependent involvement