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Background  Conjugated linoleic acid (CLA) is a group of polyunsaturated fatty acids which have been extensively studied in the past two decades. However, conjugated octadecatrienoic acid such as  cis -9, trans -11, cis -15 and  cis -9, trans -13, cis -15, recently identified, have not been extensively investigated. This work presents bioavailability and tissue incorporation of a mixture of conjugated octadecatrienoic (CLnA) acids ingested as free fatty acids (FFA) and triacylglycerols (TAG).    Results  Male Wistar rats were fed rumenic acid (RA:  cis -9, trans -11 18:2) and a CLnA mixture ( cis -9, trans -11, cis -15 18:3 and  cis -9, trans -13, cis -15 18:3) as FFA and TAG for 8 days. RA and CLnA were both totally absorbed when given as FFA as well as TAG. Both isomers of CLnA as FFA or TAG were incorporated into neutral lipids. Metabolites up to 22:6 conjugated isomers were present in liver and plasma phospholipids of rats fed the CLnA diets.    Conclusion  Finally, CLnA are as well absorbed as RA  in vivo  and their incorporation into tissues and bioconversion are similar when ingested as FFA or as TAG.

Absorption and metabolism of conjugated α-linolenic acid given as free fatty acids or triacylglycerols in rats