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Background  Oxidized low density lipoprotein plays an important role in development of foam cells in atherosclerosis. The study was focused on regulation of primary human monocyte growth and CD11b expression in presence of  Nigella sativa  oil.    Methods  Primary human monocytes were isolated from whole blood and grown at 37°C and 5% CO2 saturation for five days prior to treatment with  Nigella sativa  oil. The cells were plated and washed before treatment with ox-LDL (10 μg/ml) as positive control and combined treatment of ox-LDL (10 μg/ml) and (140 ng/ml)  Nigella sativa  oil. The growth progression was monitored every 24 hours for 3 days.    Results  Macrophages showed reduced growth in comparison to monocytes 24 hours after treatment with  Nigella sativa o il. The mean cell diameter was significantly different between untreated and treated condition in monocytes and macrophages (p < 0.001). Similarly, intracellular lipid accumulation was hindered in combined treatment with  Nigella sativa  oil. This was further supported by cell surface expression analysis, where CD11b was markedly reduced in cells treated with combination oxLDL and  Nigella sativa  oil compared to oxLDL alone. More cells differentiated into macrophage-like cells when monocytes were supplemented with oxidized LDL alone.    Conclusions  The finding provides preliminary evidence on regulation of cell growth and differentiation in monocyte and monocyte-derived macrophages by  Nigella sativa  oil. Further investigations need to be conducted to explain its mechanism in human monocyte.

Primary human monocyte differentiation regulated by Nigella sativa pressed oil