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Background   KRAS  and  BRAF  mutations appear of relevance in the genesis and progression of several solid tumor types but the co-occurrence and interaction of these mutations have not yet been fully elucidated. Using a microsatellite stable (MSS) colorectal cancer (CRC) cell line (Colo741) having mutated  BRAF  and  KRAS   WT  , we also aimed to investigate the  KRAS-BRAF  interaction. Gene expression profiles for control  KRAS   WT  ,  KRAS   G 12 V   and  KRAS   G 12 D   transfected cells were obtained after cell clone selection and RT-PCR screening. Extensive qPCR was performed to confirm microarray data.    Results  We found that the  KRAS   G 12 V   state deregulated several genes associated to cell cycle, apoptosis and nitrogen metabolism. These findings indicated a reduced survival and proliferation with respect to the  KRAS   WT   state. The  KRAS   G 12 D   state was, instead, characterized by several other distinct functional changes as for example those related to chromatin organization and cell-cell adhesion without affecting apoptosis related genes.    Conclusion  These data predict that the G12D mutation may be more likely selected in a  BRAF  mutated context. At the same time, the presence of the  KRAS   G 12 V   mutation in the cells escaping apoptosis and inducing angiogenesis via IL8 may confer a more aggressive phenotype. The present results get along with the observations that CRCs with G12V are associated with a worse prognosis with respect to the WT and G12D states and may help identifying novel CRC pathways and biomarkers of clinical relevance.

Gene expression deregulation by KRAS G12D and G12V in a BRAF V600E context