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Background  Some strains of  Bacillus licheniformis  have been improved by target-directed screening as well as by classical genetic manipulation and used in commercial thermostable α-amylase and alkaline protease production for over 40 years. Further improvements in production of these enzymes are desirable.    Results  A new strain of  B. licheniformis  CBBD302 carrying a recombinant plasmid pHY-amyL for  Bacillus licheniformis  α-amylase (BLA) production was constructed. The combination of target-directed screening and genetic recombination led to an approximately 26-fold improvement of BLA production and export in  B. licheniformis . Furthermore, a low-cost fermentation medium containing soybean meal and cottonseed meal for BLA production in shake-flasks and in a 15 liter bioreactor was developed and a BLA concentration of up to 17.6 mg per ml growth medium was attained.    Conclusion  This production level of BLA by  B. licheniformis  CBBD302(pHY-amyL) is amongst the highest levels in Gram-positive bacteria reported so far.

High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system