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Background  In an effort to identify alternate recombinant gene expression systems in  Pseudomonas fluorescens , we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon ( antABC ) and the benzoate operon ( benABCD ).    Results  The  antABC  and  benABCD  operons were identified by homology to the  Acinetobacter sp . anthranilate operon and  Pseudomonas putida  benzoate operon, and were confirmed to be regulated by anthranilate or benzoate, respectively. Fusions of the putative promoter regions to the  E. coli lacZ  gene were constructed to confirm inducible gene expression. Each operon was found to be controlled by an AraC family transcriptional activator, located immediately upstream of the first structural gene in each respective operon ( antR  or  benR ).    Conclusion  We have found the anthranilate and benzoate promoters to be useful for tightly controlling recombinant gene expression at both small (< 1 L) and large (20 L) fermentation scales.

Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions