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Background   Lactobacillus reuteri  harbors the genes responsible for glycerol utilization and vitamin B 12  synthesis within a genetic island phylogenetically related to gamma-Proteobacteria. Within this island, resides a gene ( lreu_1750 ) that based on its genomic context has been suggested to encode the regulatory protein PocR and presumably control the expression of the neighboring loci. However, this functional assignment is not fully supported by sequence homology, and hitherto, completely lacks experimental confirmation.    Results  In this contribution, we have overexpressed and inactivated the gene encoding the putative PocR in  L. reuteri . The comparison of these strains provided metabolic and transcriptional evidence that this regulatory protein controls the expression of the operons encoding glycerol utilization and vitamin B 12  synthesis.    Conclusions  We provide clear experimental evidence for assigning Lreu_1750 as PocR in  Lactobacillus reuteri . Our genome-wide transcriptional analysis further identifies the loci contained in the PocR regulon. The findings reported here could be used to improve the production-yield of vitamin B 12 , 1,3-propanediol and reuterin, all industrially relevant compounds.

Functional identification in Lactobacillus reuteri of a PocR-like transcription factor regulating glycerol utilization and vitamin B12 synthesis