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Background  Until now, the direct link between central carbon metabolism and DNA replication has been demonstrated only in  Bacillus. subtilis . Therefore, we asked if this is a specific phenomenon, characteristic for this bacterium and perhaps for its close relatives, or a more general biological rule.    Results  We found that temperature-sensitivity of mutants in particular genes coding for replication proteins could be suppressed by deletions of certain genes coding for enzymes of the central carbon metabolism. Namely, the effects of  dnaA46 (ts) mutation could be suppressed by dysfunction of  pta  or  ackA , effects of  dnaB (ts) by dysfunction of  pgi  or  pta , effects of  dnaE486 (ts) by dysfunction of  tktB , effects of  dnaG (ts) by dysfunction of  gpmA, pta  or  ackA , and effects of  dnaN159 (ts) by dysfunction of  pta  or  ackA . The observed suppression effects were not caused by a decrease in bacterial growth rate.    Conclusions  The genetic correlation exists between central carbon metabolism and DNA replication in the model Gram-negative bacterium,  E. coli . This link exists at the steps of initiation and elongation of DNA replication, indicating the important global correlation between metabolic status of the cell and the events leading to cell reproduction.

Genetic response to metabolic fluctuations: correlation between central carbon metabolism and DNA replication in Escherichia coli