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Background  Bacteria use  N -acyl homoserine lactone (AHL) molecules to regulate the expression of genes in a density-dependent manner. Several biosensors have been developed and engineered to detect the presence of all types of AHLs.    Results  In this study, we describe the usefulness of a  traI-luxCDABE -based biosensor to quickly detect AHLs from previously characterized mutants of  Burkholderia cenocepacia  and  Pseudomonas aeruginosa  in both liquid and soft-agar co-culture assays in a high-throughput manner. The technique uses a co-culture system where the strain producing the AHLs is grown simultaneously with the reporter strain. Use of this assay in liquid co-culture allows the measurement of AHL activity in real time over growth. We tested this assay with  Burkholderia cenocepacia  and  Pseudomonas aeruginosa  but it should be applicable to a broad range of gram negative species that produce AHLs.    Conclusion  The co-culture assays described enable the detection of AHL production in both  P. aeruginosa  and  B. cenocepacia  and should be applicable to AHL analysis in other bacterial species. The high-throughput adaptation of the liquid co-culture assay could facilitate the screening of large libraries for the identification of mutants or compounds that block the synthesis or activity of AHLs.

Detection of N-acyl homoserine lactones using a traI-luxCDABE -based biosensor as a high-throughput screening tool